A shift of the prooxidant-antioxidant balance in the bodies of laboratory animals at five-fold increased deuterium content in drinking diet

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Abstract

This paper presents the results of the study of the effect of water with deuterium content of 750 ppm, used for simulation of the isotopic composition of water in ice caps at the poles of Mars, on oxidative processes in the liver tissue and blood of laboratory animals. It was found that prolonged consumption of deuterium-enriched water contributed to an increase in the deuterium content in blood plasma up to 487 ppm. As a result, increased antioxidant activity in the liver tissues and blood plasma was observed. In addition, the effect of a medium that contains 487 ppm of deuterium on the secondary structure of bovine serum albumin was also investigated in a model experiment. A decrease in intensity of circular dichroism and intrinsic tryptophan fluorescence spectra was found. This indicates that there are conformational changes in the structure of this protein at a time when the content of deuterium increases in the incubation medium. The results of our research point to the need to explore further the effect of drinking diet with the increased deuterium to (from 700 to 1000 ppm) on living systems, to explain the possibility for life on Mars.

About the authors

S. V Kozin

Federal Research Centre “Southern Scientific Centre”;Kuban State University

Rostov-on-Don, Russia;Krasnodar, Russia

O. M Lyasota

Federal Research Centre “Southern Scientific Centre”;Kuban State University

Rostov-on-Don, Russia;Krasnodar, Russia

A. A Kravtsov

Federal Research Centre “Southern Scientific Centre”;Kuban State University

Rostov-on-Don, Russia;Krasnodar, Russia

E. V Chikhirzhina

Institute of Cytology, Russian Academy of Sciences

St. Petersburg, Russia

V. A Ivlev

Peoples' Friendship University of Russia

Moscow, Russia

K. A Popov

Federal Research Centre “Southern Scientific Centre”;Peoples' Friendship University of Russia

Rostov-on-Don, Russia;Moscow, Russia

A. A Dorohova

Federal Research Centre “Southern Scientific Centre”;Kuban State University

Email: 013194@mail.ru
Rostov-on-Don, Russia;Krasnodar, Russia

V. V Malyshko

Federal Research Centre “Southern Scientific Centre”;Kuban State Medical University, Ministry of Health of the Russian Federation

Rostov-on-Don, Russia;Krasnodar, Russia

A. V Moiseev

Kuban State Agrarian University

Krasnodar, Russia

References

  1. G. L. Villanueva, M. J. Mumma, R. E. Novak, et al., Science, 348 (6231), 218 (2015).
  2. J. J. Barnes, F. M. McCubbin, A. R. Santos, et al., Nat. Geosci., 13, 260 (2020).
  3. A. Basov, L. Fedulova, E. Vasilevskaya, and S. Dzhimak, Molecules. 24 (22), 4101 (2019).
  4. I. Zlatskiy, T. Pleteneva, A. Skripnikov, et al., Molecules, 25 (18), 4173 (2020).
  5. М. И. Быков, С. С. Джимак, А. А. Басов и др., Вопросы питания, 84 (4), 89 (2015).
  6. В. И. Лобышев и Л. П. Калиниченко, Изотопные эффекты D2O в биологических системах (Наука, М., 1978).
  7. В. И. Лобышев, В. А. Твердислов Л. В. Яковенко и др., Биофизика, 2, 390 (1978).
  8. В. И. Лобышев, Ю. Фогель, Л. В. Яковенко и др., Биофизика, 4, 595 (1982).
  9. В. И. Лобышев, Биофизика, 4, 666 (1983).
  10. V. I. Lobyshev, Int. J. High Dilution Res., 17 (2), 12 (2018).
  11. X. Zhang, J. Wang, and R. A. Zubarev, Mol. Cell. Proteomics, 19 (11), 1790 2020.
  12. A. Basov, L. Fedulova, M. Baryshev, and S. Dzhimak, Nutrients, 11 (8), 1903 (2019).
  13. S. V. Kozin, E. I. Zlischeva, L. V. Shurygina, et al., Biophysics, 65 (6), 1017 (2020).
  14. S. Hou, P. Chen, J. He, et al., Redox Biol., 57 (11), 102472 (2022).
  15. A. Basov, M. Drobotenko, A. Svidlov, et al., Molecules, 25 (16), 3753 (2020).
  16. A. Syroeshkin, T. Pleteneva, E. Uspenskaya, et al., Chem. Engineer. J., 377, 1 (2019).
  17. R. Rehakova, J. Klimentova, M. Cebova, et al., Physiol. Res., 65 (3), 401 (2016)
  18. A. A. Svidlov, M. I. Drobotenko, A. A. Basov, et al., Physics of Wave Phenomena, 29 (2), 180 (2021)
  19. A. V. Syroeshkin, N. V. Antipova, A. V. Zlatska, et al., J. Trace Elements Med. Biol., 50, 629 (2018).
  20. N. V. Lobysheva, S. V. Nesterov, Y. A. Skorobogatova, et al., Biophysics, 65 (2), 272 (2020).
  21. A. B. Lisicin, M. G. Barishev, A. A. Basov, et al., Biophysics, 59 (4), 620 (2014).
  22. K. Yavari and L. Kooshesh, Nutrition and Cancer, 71 (6), 1019 (2019).
  23. A. Kravtsov, S. Kozin, A. Basov, et al., Molecules, 27 (1), 243 (2022).
  24. S. Kozin, A. Kravtsov, M. Baryshev, et al, Molecules, 26 (7), 2036 (2021).
  25. M. G. Baryshev, A. A. Basov, S. N. Bolotin, et al., Bull.Russ. Acad. Sci.: Physics, 76 (12), 1349 (2012).
  26. N. V. Yaglova, S. S. Obernikhin, E. P. Timokhina, et al., Bull. Exp. Biol. Med., 171 (2), 262 (2021).
  27. X. Xie and R. A. Zubarev, Sci. Rep., 5 (9215), 1 (2015).
  28. S. S. Dzhimak, M. G. Barishev, A. A. Basov, et al., Biophysics, 59 (4), 614 (2014).
  29. T. Halenova, I. Zlatskiy, A. Syroeshkin, et al., Molecules, 25 (1), 23 (2020).
  30. N. V. Yaglova, S. S. Obernikhin, E. P. Timokhina, et al., Bull. Exp. Biol. Med., 171 (5), 572 (2021).
  31. А. А. Басов, М. И. Быков, М. Г. Барышев и др., Вопросы питания, 83 (5), 43 (2014).
  32. G. Pizzino, N. Irrera, M. Cucinotta, et al., Oxid Med. Cell. Longev., 2017, (2017).
  33. M. S. Shchepinov, Rejuvenation Res., 10 (1), 47 (2007).
  34. I. M. Bykov, A. A. Basov, V. V. Malyshko, et al., Bull. Exp. Biol. Med., 163 (2), 268 (2017).
  35. Guide for the Care and Use of Laboratory Animals: Eighth Edition (The National Academies Press, Washington, DC, 2011).
  36. С. В. Козин, А. А. Кравцов, С. В. Кравченко и др., Вопросы питания, 90 (2), 63 (2021).
  37. S. S. Dzhimak, A. A. Basov, G. F. Kopytov, et al., Rus. Phys. J., 58 (7), 923 (2015).
  38. М. М. Туйгунов, Р. Р. Фархутдинов и Ю. А. Медведев, Мед. иммунология, 8 (2-3), 427 (2006).
  39. S. V. Kozin, A. A. Kravtsov, A. A. Elkina, et al., Biophysics, 64 (2), 272 (2019).
  40. I. R. Ilyasov, V. L. Beloborodov, I. A. Selivanova, et al., Int. J. Mol. Sci., 21 (3), 1131 (2020).
  41. A. A. Basov, A. A. Elkina, S. S. Dzhimak, et al., Biol. Bull., 46 (6), 531 (2019).
  42. L. V. Shurygina, A. A. Kravtsov, E. I. Zlishcheva, et al., Neurochem. J., 11 (3), 250 (2017).
  43. А. М. Поляничко, Н. В. Михайлов, Н. М. Романов и др., Цитология, 58 (9), 707 (2016).
  44. О. В. Степаненко, И. М. Кузнецова, К. К. Туроверов и др., Цитология, 47 (11), 988 (2005).
  45. M. L. F. Estep and T. C. Hoering, Carnegie Institution of Washington, 78, 652 (1979).
  46. S. S. Dzhimak, M. I. Drobotenko, A. A. Basov, et al., Dokl. Biochem. Biophys., 483, 359 (2018).
  47. S. V. Kozin, A. A. Kravtsov, A. V. Churkina et al., Biophysics, 65 (2), 195 (2020).
  48. T. L. Rosenberry, Molecules, 25 (19), 4412 (2020).
  49. N. Frankowska, K. Lisowska, J. M. Witkowski, Front Aging, 927630 (2022).
  50. E. Amzallag, E. Hornstein, Cells, 11 (15), 2415 (2022).
  51. C. Hu, J. Yang, Z. Qi, et al., MedComm., 2 (3), 381 (2020).
  52. H. Saibil, Nature Rev. Mol. Cell Biol., 14 (10), 630 (2013).

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