Cellular response to exposure to ionizing radiation and light in the presence of a photosensitizer

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Resumo

This study has been conducted to investigate the effects of X-ray irradiation in combination with photodynamic therapy on human lung adenocarcinoma cell line A549 used as a lung cancer model. It has been established that the concomitant use of these two types of radiation leads to much lower survival rate of tumor cells than that observed after the sequential use of these types of radiation or exposure to each type of radiation measured separately. For different combinations of the two mentioned types of radiation, the mRNA expression levels of some genes involved in cell cycle regulation (p21, DINOL), apoptosis (BAX, PUMA), and DNA repair (PARP1, OGG1, Rad51, Lig4) were determined in cells. The highest level of expression after exposure to different combinations of radiation was observed for the lncRNA DINOL gene. The increased expression of the BAX and PUMA genes indicates the development of apoptosis. The results obtained confirm that there is synergism in X-ray radiation and photodynamic therapy due to the concomitant use of these types of radiation.

Sobre autores

S. Akulinichev

Institute for Nuclear Research, Russian Academy of Sciences;B.V. Petrovsky Russian Research Center of Surgery

Email: akulinic@inr.ru
Troitsk, Moscow, Russia;Moscow, Russia

S. Glukhov

Institute for Nuclear Research, Russian Academy of Sciences;Institute for Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: serglukhovmb@gmail.com
Troitsk, Moscow, Russia;Pushchino, Moscow Region, Russia

A. Efremenko

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Moscow, Russia

D. Kokontsev

Institute for Nuclear Research, Russian Academy of Sciences;B.V. Petrovsky Russian Research Center of Surgery

Troitsk, Moscow, Russia;Moscow, Russia

E. Kuznetsova

Institute for Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

V. Martynova

Institute for Nuclear Research, Russian Academy of Sciences

Troitsk, Moscow, Russia

A. Feofanov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences;Lomonosov Moscow State University

Moscow, Russia

I. Yakovlev

Institute for Nuclear Research, Russian Academy of Sciences;B.V. Petrovsky Russian Research Center of Surgery

Troitsk, Moscow, Russia;Moscow, Russia

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