Effect of estradiol on the sensitivity of Staphylococcus aureus 209p to tetracycline in vitro

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Resumo

In this work, the combined inhibitory effect of the hormone estradiol and the antibiotic tetracycline against the type strain of Staphylococcus aureus 209 P is demonstrated for the first time. The combined addition of the hormone and antibiotic leads to a significant increase in the doubling time of the culture compared to samples with tetracycline (0.05 μg/ml) without the addition of the hormone. The combined effect of the compounds is most pronounced against the planktonic culture at the initial stages of growth, but at the same time it is also preserved against biofilms. In almost all cases, estradiol has the greatest effect at concentrations exceeding the physiological one (2.2 × 10−10 M) by one and two orders of magnitude.

Sobre autores

N. Loginova

S.N. Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: aegopodium102011@gmail.com
Moscow, 119071, Russia

A. Metkina

S.N. Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: aegopodium102011@gmail.com
Moscow, 119071, Russia

A. Gannensen

S.N. Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: aegopodium102011@gmail.com
Moscow, 119071, Russia

S. Martyanov

S.N. Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: aegopodium102011@gmail.com
Moscow, 119071, Russia

Bibliografia

  1. Ганнесен А.В., Зиганьшин Р.Х., Овчарова М.А., Мосолова А.М., Логинова Н.А., Дювенжи Е.В., Неволина Е.Д., Мартьянов С. В., Плакунов В. К. Изменения белковых профилей планктонных культур и биопленок Staphylococcus epidermidis, выращенных в анаэробных условиях в присутствии гормона CNP // Микробиология. 2024. Т. 93. С. 757‒772. https://doi.org/10.31857/S0026365624060072
  2. Gannesen A. V., Ziganshin R. H., Ovcharova M. A., Mosolova A. M., Loginova N. A., Diuvenji E. V., Nevolina E. D., Mart’yanov S.V., Plakunov V. K. Changes in the protein profiles of Staphylococcus epidermidis planktonic cultures and biofilms under anoxic conditions in the presence of the CNP hormone // Microbiology (Moscow). 2024. V. 93. P. 799‒811. https://doi.org/10.1134/S0026261724607188
  3. Дювенжи Е. В., Соловьев И. Д., Сухачева М. В., Неволина Е. Д., Овчарова М. А., Логинова Н. А., Мосолова А. М., Мартьянов С. В., Плакунов В. К., Ганнесен А. В. Модуляция активности азитромицина в отношении моновидовых и бинарных биопленок Staphylococcus aureus и Kytococcus schroeteri гормоном норадреналином // Микробиология. 2024. Т. 93. С. 732‒744. https://doi.org/10.31857/S0026365624060058
  4. Diuvenji E. V., Soloviev I. D., Sukhacheva M. V., Nevolina E. D., Ovcharova M. A., Loginova N. A., Mosolova A. M., Mart’yanov S.V., Plakunov V. K., Gannesen A. V. Modulation of azithromycin activity against single-species and binary biofilms of Staphylococcus aureus and Kytococcus schroeteri by norepinephrine // Microbiology (Moscow). 2024. V. 93. P. 777–787. https://doi.org/10.1134/S0026261724606560
  5. Мартьянов С. В., Ганнесен А. В., Плакунов В. К. Новый метод получения моновидовых и бинарных культур Staphylococcus spp., выращиваемых в альгинатном геле, и исследование действия на них активных соединений на примере катехоламинов // Бюллетень экспериментальной биологии и медицины. 2024. Т. 177 (4). С. 524‒528.
  6. Bertuccini L., Russo R., Iosi F., Superti F. Effects of Lactobacillus rhamnosus and Lactobacillus acidophilus on bacterial vaginal pathogens // Int. J. Immunopathol. Pharmacol. 2017. V. 30. P. 163‒167. https://doi.org/10.1177/0394632017697987
  7. Byrd A. L., Belkaid Y., Segre J. A. The human skin microbiome // Nat. Rev. Microbiol. 2018. V. 6. P. 143‒155. https://doi.org/10.1038/nrmicro.2017.157
  8. Cheung G. Y.C., Bae J. S., Otto M. Pathogenicity and virulence of Staphylococcus aureus // Virulence. 2021. V. 12. P. 547‒569. https://doi.org/10.1080/21505594.2021.1878688
  9. Emaneini M., Bigverdi R., Kalantar D., Soroush S., Jabalameli F., Noorazar Khoshgnab B., Asadollahi P., Taherikalani M. Distribution of genes encoding tetracycline resistance and aminoglycoside modifying enzymes in Staphylococcus aureus strains isolated from a burn center // Ann. Burns Fire Disasters. 2013. V. 26. № 2. P. 76‒80.
  10. Fournière M., Latire T., Souak D., Feuilloley M. G.J., Bedoux G. Staphylococcus epidermidis and Cutibacterium acnes: two major sentinels of skin microbiota and the influence of cosmetics // Microorganisms. 2020. V. 8. Art. 1752. https://doi.org/10.3390/microorganisms8111752
  11. Gannesen A. V., Lesouhaitier O., Racine P. J., Barreau M., Netrusov A. I., Plakunov V. K., Feuilloley M. G.J. Regulation of monospecies and mixed biofilms formation of skin Staphylococcus aureus and Cutibacterium acnes by human natriuretic peptides // Front. Microbiol. 2018. V. 9. Art. 2912. https://doi.org/10.3389/fmicb.2018.02912
  12. Gjertsson I., Lagerquist M. K., Kristiansson E., Carlsten H., Lindholm C. Estradiol ameliorates arthritis and protects against systemic bone loss in Staphylococcus aureus infection in mice // Arthritis Res. Ther. 2012. V. 14. P. 76–84. https://doi.org/10.1186/ar3799
  13. Grice E. A. The intersection of microbiome and host at the skin interface: genomic- and metagenomic-based insights // Genome Res. 2015 V. 25. P. 1514‒1520. https://doi.org/10.1101/gr.191320.115
  14. Kalaycı-Yüksek F., Gümüş D., Güler V., Uyanık-Öcal A., Anğ-Küçüker M. Progesterone and estradiol alter the growth, virulence and antibiotic susceptibilities of Staphylococcus aureus // New Microbiol. 2023. V. 46. P. 43‒51.
  15. Kim Y. S., Kim H. S. Tetracyclines revisited: tetracyclines in the field of dermatology // Dermatology. 2024. V. 240. P. 844‒858. https://doi.org/10.1159/000542006
  16. Kiseleva A. A., Solovyeva T. V., Ovcharova M. A., Geras’kina O.V., Mart’yanov S.V., Cherdyntseva T. A., Danilova N. D., Zhurina M. V., Botchkova E. A., Feofanov A. V., Plakunov V. K., Gannesen A. V. Effect of β-estradiol on mono- and mixed-species biofilms of human commensal bacteria Lactobacillus paracasei AK508 and Micrococcus luteus C01 on different model surfaces // Coatings. 2022. V. 12. Art. 436. https://doi.org/10.3390/coatings12040436
  17. Mart’yanov S.V., Botchkova E. A., Plakunov V. K., Gannesen A. V. The impact of norepinephrine on mono-species and dual-species staphylococcal biofilms. // Microorganisms. 2021. V. 9. Art. 820. https://doi.org/10.3390/microorganisms9040820
  18. Medina-Estrada I., López-Meza J.E., Ochoa-Zarzosa A. Anti-inflammatory and antimicrobial effects of estradiol in bovine mammary epithelial cells during Staphylococcus aureus internalization // Mediators Inflamm. 2016. V. 2016. P. 16–32. https://doi.org/10.1155/2016/6120509
  19. Mittelstaedt R., Kanjilal S., Helekal D., Robbins G. K., Grad Y. H. Staphylococcus aureus tetracycline resistance and co-resistance in a doxy-pep-eligible population // J. Infect. Dis. 2025. V. 231. P. 708‒712. https://doi.org/10.1093/infdis/jiae634
  20. Ning E., Turnbull G., Clarke J., Picard F., Riches P., Vendrell M., Graham D., Wark A. W., Faulds K., Shu W. 3D bioprinting of mature bacterial biofilms for antimicrobial resistance drug testing // Biofabrication. 2019. V. 11. № 4. ID045018. https://doi.org/10.1088/1758-5090/ab37a0
  21. Souza C. L.S.E., Barbosa C. D., Coelho H. I.L.N., Santos Júnior M. N., Barbosa E. N., Queiroz É. C., Teles M. F., Dos Santos D. C., Bittencourt R. S., Soares T. J., Oliveira M. V., Timenetsky J., Campos G. B., Marques L. M. Effects of 17β-estradiol on monocyte/macrophage response to Staphylococcus aureus: an in vitro study // Front. Cell Infect. Microbiol. 2021. V. 11. Art. 701391. https://doi.org/10.3389/fcimb.2021.701391
  22. Spiesz E. M., Yu K., Lehner B. A.E., Schmieden D. T., Aubin-Tam M.E., Meyer A. S. Three-dimensional patterning of engineered biofilms with a do-it-yourself bioprinter // J. Vis. Exp. 2019. Art. 147. https://doi.org/10.3791/59477
  23. Taneja V. Sex hormones determine immune response // Front. Immunol. 2018. V. 9. Art. 1931. https://doi.org/10.3389/fimmu.2018.01931
  24. O’Toole GA. Microtiter dish biofilm formation assay // J. Vis. Exp. 2011. V. 47. Art. 2437. https://doi.org/10.3791/2437
  25. Tsuru A., Hamazaki Y., Tomida S., Ali M. S., Komura T., Nishikawa Y., Kage-Nakadai E. Nonpathogenic Cutibacterium acnes confers host resistance against Staphylococcus aureus // Microbiol. Spectr. 2021. V. 9. Art. e0056221. https://doi.org/10.1128/Spectrum.00562-21
  26. Yan L., Rui C., Zhuang B., Liu X., Luan T., Jiang L., Dong Z., Wang Q., Wu A., Li P., Wang X., Zeng X. 17β-estradiol mediates Staphylococcus aureus adhesion in vaginal epithelial cells via estrogen receptor α-associated signaling pathway // Curr. Microbiol. 2023. V. 80. Art. 391. https://doi.org/10.1007/s00284-023-03488-6

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