Changes in the Protein Composition of the Exiguobacterium sibiricum Membrane with Decreasing Temperature: a Proteomic Analysis

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Resumo

For the first time, the proteins of the membrane fraction isolated from the psychrotrophic bacterium Exiguobacterium sibiricum were analyzed using the label-free quantitative chromatography-mass spectrometry. Comparison of the samples from cells cultured at 10°C and at room temperature revealed significant differences in the content of many proteins involved in important physiological processes, including DNA and RNA binding proteins, membrane transporters, proteins providing protection against osmotic and oxidative stress, and others. The results of the work contribute to understanding of mechanisms of the processes occurring in the cell membranes of psychrotrophic bacteria at low temperatures, and complement existing ideas about the adaptation strategies of microorganisms living in permafrost deposits. The data obtained can also be used to search for potential biocatalysts with activity at low temperatures.

Sobre autores

L. Petrovskaya

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS; Moscow Institute of Physics and Technology

Email: lpetr65@yahoo.com
Moscow, Russia; Dolgoprudny, Russia

R. Ziganshin

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, Russia

E. Kryukova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, Russia

E. Spirina

Institute of Physical, Chemical and Biological Problems of Soil Science, RAS

Pushchino, Russia

E. Rivkina

Institute of Physical, Chemical and Biological Problems of Soil Science, RAS

Pushchino, Russia

S. Siletsky

Research Institute of Physical and Chemical Biology named after A.N. Belozersky, Lomonosov Moscow State University; Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics

Moscow, Russia; Moscow, Russia

D. Dolgikh

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS; Lomonosov Moscow State University, Faculty of Biology

Moscow, Russia; Moscow, Russia

M. Kirpichnikov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS; Lomonosov Moscow State University, Faculty of Biology

Moscow, Russia; Moscow, Russia

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