Interaction of Lyophilic Zinc(II) Porphyrins with Bovine Serum Albumin

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Palladium-catalyzed heterylation of monobromophenyl-substituted zinc(II) porphyrin with small heterocycles (benzothiazole, benzoxazole, and N-methylbenzimidazole) was carried out. As a result, unsymmetrical heterylphenyl-substituted zinc(II) porphyrins soluble in organic solvents were obtained. The interaction of heteryl-substituted zinc(II) porphyrins with alpha-helical proteins was studied by spectral methods using bovine serum albumin in aqueous organic solvents. It was found that the titration of the zinc(II) porphyrins with albumin in a sodium phosphate buffer involves a number of equilibria including complexation and aggregation. In the case of porphyrins containing N-methylbenzimidazole and benzoxazole residues, self-aggregation processes initiated by absorption of organic solvent molecules by the protein predominate. It was found that more hydrophobic nature of zinc(II) porphyrin with benzothiazole residue promotes the complex formation with the protein. The photochemical properties of zinc(II) porphyrin with a benzothiazole residue, capacity for the photooxidation of the alpha-helical protein, and the high affinity of protein to this porphyrin make it a promising candidate for the potential applicability for photodynamic inactivation.

全文:

受限制的访问

作者简介

O. Koifman

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences; Ivanovo State University of Chemistry and Technology

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo; Ivanovo

N. Lebedeva

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

E. Yurina

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

编辑信件的主要联系方式.
Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

Yu. Gubarev

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

S. Syrbu

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences; Ivanovo State University of Chemistry and Technology

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo; Ivanovo

A. Kiselev

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo

M. Lebedev

G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences; Ivanovo State University of Chemistry and Technology

Email: yurina_elena77@mail.ru
俄罗斯联邦, Ivanovo; Ivanovo

参考

  1. Mancuso G., Midiri A., Gerace E. et al. // Pathogens. 2021. V. 10. P. 1310. https://doi.org/10.3390/pathogens10101310
  2. Urban-Chmiel R., Marek A., Stepie´n-Pysniak D. et al. //Antibiotics. 2022. V. 11. № 8. P. 1079. https://doi.org/10.3390/antibiotics11081079
  3. Ten threats to global health in 2019 // World Health Organization. 2019. V. 22. P. 2021. https://www.who.int/news-room/spotlight/ten-threats-to-global-health-in-2019
  4. Kenawy E.R., Worley S.D., Broughton R. // Biomacromolecules. 2007. V. 8. № 5. P. 1359. https://doi.org/10.1021/bm061150q
  5. Stojiljkovic I., Evavold B.D., Kumar V. // Expert opinion on investigational drugs. 2001. V. 10. № 2. P. 309. https://doi.org/10.1517/13543784.10.2.309
  6. König K., Teschke M., Sigusch B. et al. // Cellular Mol. Biol. 2000. V. 46. № 7. P. 1297.
  7. Lebedeva N.S., Gubarev Y.A., Koifman M.O. et al. // Molecules. 2020. V.25. P. 4368 https://www.mdpi.com/1420-3049/25/19/4368
  8. Lebedeva N.S., Koifman O.I. // Russ. J. Bioorg. Chemi. 2022. V. 48. №. 1. P. 1. https://doi.org/10.1134/S1068162022010071
  9. Koifman O.I., Ageeva Т.А., Beletskaya I.P. et al. // Macroheterocycles. 2020. V. 13. № 4. P. 183. https://doi.org/10.6060/mhc200814k
  10. Koifman O.I., Ageeva T.A., Kuzmina N. S. et al. // Macroheterocycles. 2022. V. 15. № 4. P. 207. https://doi.org/10.6060/mhc224870k
  11. Kiselev A.N., Lebedev M.A., Syrbu S.A. et al. // Russ. Chem. Bull. V. 71. № 12. P. 2691. https://doi.org/10.1007/s11172-022-3698-5
  12. Lebedeva N.S., Yurina E.S., Gubarev Y.A. et al. //Mendeleev Commun. 2017. V. 27. № 1. P. 47-49. https://doi.org/10.1016/j.mencom.2017.01.014
  13. Lebedeva N.S., Yurina E.S., Gubarev Y.A. et al. // J. Inclus. Phenom. Macrocycl. Chem. 2019. V. 95. № 3–4. P. 199. https://doi.org/10.1007/s10847-019-00947-1.
  14. Yurina E.S., Gubarev Y.A., Kiselev A.N. et al. // Mendeleev Commun. 2020. V. 30. № 2. P. 211. https://doi.org/10.1016/j.mencom.2020.03.027
  15. Lebedeva N.S., Yurina E.S., Gubarev Y.A. et al. // Spectrochim. Acta. A.2021. V. 246. P. 118975. https://doi.org/10.1016/j.saa.2020.11897.5
  16. Lebedeva N.S., Yurina E.S., Gubarev Y.A. et al. // Russ. J. Gen. Chem. 2019. V. 89. P. 565. https://doi.org/10.1134/S1070363219030368
  17. Ogunsipe A., Nyokong T. // J. Mol. Struct. 2004. V.689. P.89. https://doi.org/10.1016/j.molstruc.2003.10.024
  18. Ormond A.B., Freeman H.S. // Dyes Pigments. 2013. V. 96. № 2. P. 440. https://doi.org/10.1016/j.dyepig.2012.09.011
  19. Figueiredo T.L.C. Johnstone, R. A., Sørensen, A.M.S. et al. // Photochem. Photobiol. 1999. V. 69. № 5. P. 517. https://doi.org/10.1111/j.1751-1097.1999.tb03322.x
  20. Lebedeva N.Sh., Mal´kova E.A., Gubarev Yu.A., et al. Uch. Zap. Petrozavodsk. Gos. Univ. 2014 vol. 1. № 8 (145). P. 12.
  21. Rothemund P., Menotti A.R. // J. Am. Chem. Soc. 1948. V. 70. № 5. P. 1808. https://doi.org/10.1021/ja01185a047
  22. Kiselev A.N., Syrbu S.A., Lebedeva N.Sh. et.al. // Inorganics. 2022. V. 10. № 63. P. 1. https://doi.org/10.3390/inorganics10050063
  23. Akins D.L., Özçelik S., Zhu H. R. et al. // J. Phys Chem. 1996. V. 100. № 34. P. 14390. https://doi.org/10.1021/jp961013v
  24. Ghosh M., Nath S., Hajra A. et al. // J. Lumin. 2013. V. 141. P. 87. https://doi.org/10.1016/j.jlumin.2013.03.025

补充文件

附件文件
动作
1. JATS XML
下载
2. Scheme 1. Synthesis of asymmetric heteryl-substituted zinc(II)porphyrins

下载 (182KB)
索引源数据
3. Fig. 1. ZnPorO ESP (8.210-6 M) (a) and its fluorescence spectrum (b) in DMFA (dotted line) and PBS-DMFA (0.19 M) (solid line).

下载 (165KB)
索引源数据
4. Fig. 2. ESP ZnPorS (4.9910-6 M) (a) and ZnPorN (6���10-6 M) (b) in PBS-DMFA –0.19 M) during titration of BSA (0-6���10-6 M.

下载 (146KB)
索引源数据
5. Fig. 3. Corrected fluorescence spectra of BSA (2.2410-5 M) during ZnPorS titration (0-7.82���10-6 M) in PBS–DMFA (0.19 M), taking into account the absorption of ZnPorS.

下载 (114KB)
索引源数据
6. Fig. 4. Constants of the observed rate of photooxidation of BSA in the presence of ZnPorX under irradiation with 425 nm light, estimated as the ratio of BSA fluorescence before irradiation (lnF0) to BSA fluorescence (Fi) under irradiation.

下载 (72KB)
索引源数据

版权所有 © Российская академия наук, 2024