Use of Metabolic Inert Mycobacterium abscessus Cells to Study the Efficiency Of Drugs

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Abstract

We investigated the effectiveness of antibiotics (amikacin, bedaquiline, linezolid, moxifloxacin, rifampicin) on metabolically inert M. abscessus obtained under conditions of potassium deficiency in vitro. It was found that bedaquiline led to a significant decrease in the ability of bacteria to form colonies on solid media, but did not lead to their death, since it was shown that during cultivation in a liquid medium, they reverted to a state of active division and growth. Moxifloxacin had a bactericidal effect against metabolically inert bacteria, irreversibly and significantly reducing the number of viable cells in culture, which emphasizes the effectiveness of its use for the treatment of infections caused by M. abscessus.

About the authors

B. A. Martini

A.N. Bach Institute of Biochemistry, Research Center of Biotechnologies Russian Academy of Sciences

Email: elenasalina@yandex.ru
Russia, 119071, Moscow

E. G. Salina

A.N. Bach Institute of Biochemistry, Research Center of Biotechnologies Russian Academy of Sciences; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: elenasalina@yandex.ru
Russia, 119071, Moscow; Russia, 117997, Moscow

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Copyright (c) 2023 Б.А. Мартини, Е.Г. Салина