Определение полифенольного комплекса в Reynoutria japonica Houtt. методом тандемной масс-спектрометрии

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Abstract

Целью данной работы являлось уточнение метаболомного состава экстрактов, в частности наличие полифенольного комплекса в экстрактах лекарственного растения Reynoutria japonica Houtt., принадлежащего к семейству Polygonaceae. Для идентификации целевых аналитов в экстрактах использовалась тандемная масс-спектрометрия листьев и стеблей. Всего идентифицировано 31 химическое соединение, из них 18 соединений представляют полифенольный комплекс. В приложение к идентифицированным вторичным метаболитам некоторые соединения были обнаружены впервые, в частности полифенольные соединения: дигидрохалкон аспалатин, кумарин умбеллиферон, лигнан сирингарезинол, а также флавоны формононетин и гарденин Б.

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М. П. Разгонова

Федеральное государственное бюджетное научное учреждение “Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова”

Author for correspondence.
Email: m.razgonova@vir.nw.ru
Russian Federation, Санкт-Петербург

E. И. Черевач

Федеральное государственное автономное образовательное учреждение высшего образования “Дальневосточный федеральный университет”

Email: m.razgonova@vir.nw.ru

Передовая инженерная школа “Институт биотехнологий, биоинженерии и пищевых систем”

Russian Federation, Владивосток

Н. С. Кириленко

Федеральное государственное бюджетное научное учреждение “Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова”

Email: m.razgonova@vir.nw.ru
Russian Federation, Санкт-Петербург

E. Н. Демидова

Федеральное государственное бюджетное научное учреждение “Федеральный исследовательский центр Всероссийский институт генетических ресурсов растений им. Н.И. Вавилова”

Email: m.razgonova@vir.nw.ru
Russian Federation, Санкт-Петербург

К. С. Голохваст

Федеральное государственное бюджетное научное учреждение “Сибирский федеральный научный центр агробиотехнологий Российской академии наук”

Email: m.razgonova@vir.nw.ru
Russian Federation, Краснообск

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Supplementary files

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2. Fig. 1. Distribution of the yield density of biologically active substances in tandem mass spectrometry of the analyzed target analytes of Reynoutria japonica extract.

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3. Fig. 2. Mass spectrum of chlorogenic acid from Reynoutria japonica leaf extract. At the top is the MS scan in the range of 100–1700 m/z, at the bottom are the fragmentation spectra (from top to bottom): MS2 of the protonated ion of chlorogenic acid (355.11 m/z, red diamond), MS3 of the fragment 355.11→163.13 m/z and MS4 of the fragment 355.19→163.13→145.16 m/z.

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4. Fig. 3. Pathway of fragmentation of the chlorogenic acid molecule.

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5. Fig. 4. Mass spectrum of formononetin from Reynoutria japonica stem extract. At the top is the MS scan in the range of 100–1700 m/z, at the bottom are the fragmentation spectra (from top to bottom): MS2 of the protonated ion of formononetin (269.29 m/z, red diamond), MS3 of the fragment 269.29→213.22 m/z and MS4 of the fragment 269.29→213.22→196.21 m/z.

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6. Fig. 5. Mass spectrum of quercitrin from Reynoutria japonica leaf extract. At the top is the MS scan in the range of 100–1700 m/z, at the bottom are the fragmentation spectra (from top to bottom): MS2 of the protonated quercitrin ion (447.38 m/z, red diamond), MS3 of the fragment 447.38→301.18 m/z and MS4 of the fragment 447.38→301.18→179.19 m/z.

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7. Fig. 6. Venn diagram of tentatively identified chemical compounds of Reynoutria japonica.

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8. Fig. 7. Mass spectrum of 1,4,8-trihydroxyanthraquinone from Reynoutria japonica leaf extract. At the top is the MS scan in the range of 100–1700 m/z, at the bottom are the fragmentation spectra (from top to bottom): MS2 of the protonated ion of 1,4,8-trihydroxyanthraquinone (256.17 m/z, red diamond), MS3 of the fragment 256.17→210.18 m/z and MS4 of the fragment 256.17→210.18→181.93 m/z.

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