Protein and amino acid fluorescence analysis as a method for evaluating bactericidal effectiveness

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The degradation of albumin and its constituent amino acids tryptophan, tyrosine, and phenylalanine under the influence of corona discharge cold plasma, pulsed radiation of hot plasma, and 253.7-nm UV light from a mercury lamp has been studied. The concentration of products before and after treatment was determined by measuring the fluorescence yield. The reaction mechanisms leading to protein degradation are oxidation, nitration, and reduction of disulfide bonds. The degree of protein and amino acid degradation is compared with the effect of hydrogen peroxide. The possibility of assessing the bactericidal effectiveness based on fluorescence yield is discussed.

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Sobre autores

I. Piskarev

Moscow State University

Autor responsável pela correspondência
Email: i.m.piskarev@gmail.com

Skobeltsyn Research Institute of Nuclear Physics

Rússia, Moscow

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2. Fig. 1. Corona electric discharge generator. 1 – treated liquid, 2 – discharge electrodes, 3 – RC matrix, 4 – high voltage source.

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3. Fig. 2. Hot plasma pulse radiation generator IR10. 1 – cuvette with treated liquid, 2 – spark discharge module, 3 – power supply module; C1 – discharge capacitor 2200 pf; R1 = R2 = 330 kOhm – reflected wave attenuator; R3 – ballast resistance 8 mOhm, HV – high voltage source 11 sq. m.

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4. 3. The dependence of the relative decrease in amino acid concentration after treatment (C / C0) on the dose of UV lamp irradiation (J / 10 ml), l = 253.7 nm.

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