Enviar artigo por via de e-mail Rapid detection of A-type botulinum toxin using an aptasensor and SERS
- Autores: Ambartsumyan O.A.1, Brovko A.M.1
-
Afiliações:
- Moscow Institute of Physics and Technology
- Edição: Volume 88, Nº 2 (2024)
- Páginas: 219-226
- Seção: New Materials and Technologies for Security Systems
- URL: https://cardiosomatics.ru/0367-6765/article/view/654753
- DOI: https://doi.org/10.31857/S0367676524020097
- EDN: https://elibrary.ru/RSHEGU
- ID: 654753
Citar
Texto integral
Acesso aberto
Acesso está concedido
Acesso é pago ou somente para assinantes
Resumo
We described the development of a biosensor for the rapid and sensitive detection of botulinum toxin type A. The sensor is a SERS substrate with an optimized concentration of labeled aptamers, immobilized on its surface. It allows the detection of botulinum toxin type A with a detection limit of 2.4 ng/ml in 1 hour.
Palavras-chave
Texto integral
Sobre autores
O. Ambartsumyan
Moscow Institute of Physics and Technology
Autor responsável pela correspondência
Email: ambartsumian.oa@mipt.ru
Rússia, Moscow
A. Brovko
Moscow Institute of Physics and Technology
Email: ambartsumian.oa@mipt.ru
Rússia, Moscow
Bibliografia
- Pirazzini M., Rossetto O., Eleopra R., Montecuccco C. // Pharmacol. Rev. 2017. V. 69. P. 200.
- Smith T.J., Hill K.K., Raphael B.H. // Res. Microbiol. 2015. V. 166. P. 290.
- Rummel A. // Toxicon. 2015. V. 107. P. 9.
- Tian D., Zheng T. // PLoS One. 2014. V. 9. P. 1.
- Hill K.K., Smith T.J., Helma C.H. et al. // J. Bacteriol. 2007. V. 189. No. 3. P. 818.
- Strotmeier J., Willjes G., Binz T., Rummel A. // FEBS Lett. 2012. V. 586. P. 310.
- Kavalali E.T. // Nature. Rev. Neurosci. 2015. V. 16. P. 5.
- Kamińska A., Winkler K., Kowalska A. et al. // Sci. Rеports. 2017. V. 7. Art. No. 10656.
- Lim C.Y., Granger J.H., Porter M. // Analyt. Chim. Acta X. 2019. V. 1. Art. No. 100002.
- Kim K., Choi N., Jeon J.H. et al. // Sensors. 2019. V. 19. Art. No. 4081.
- Yoo H., Jo H., Oh S.S. // Mater. Adv. 2020. V. 1. P. 2663.
- Frevert J. // Drugs R D. 2010. V. 10. P. 67.
Arquivos suplementares
Arquivos suplementares
Ação
1.
JATS XML
2.
Fig. 1. Schematic diagram of the experiment
Baixar (153KB)
3.
Fig. 2. Ratio of SERS signal intensity of experiment and control
Baixar (111KB)
4.
Fig. 3. Distribution of SERS signal intensity from the aptamer solution to BoNT with concentration of 35.0 nM on the substrate
Baixar (167KB)
5.
Fig. 4. SERS signal intensity distribution from the aptamer solution to BoNT with 17.5 nM concentration on the substrate
Baixar (121KB)
6.
Fig. 5. SERS signal intensity distribution from the aptamer solution to BoNT with 8.8 nM concentration on the substrate
Baixar (134KB)
7.
Fig. 6. SERS signal intensity distribution of experiment and control for BoNT A (2.4 ng/ml) detection using a solution of aptamers with a concentration of 17.5 nM on the substrate
Baixar (197KB)
8.
Fig. 7. Distribution of SERS intensity of experiment and control in the determination of IgGHum (24 ng/ml) using a solution of aptamers to BoNT with a concentration of 17.5 nM on the substrate
Baixar (170KB)
9.
Fig. 8. Distribution of SERS intensity of the experiment and control in the determination of BoNT A (2.4 ng/ml) using a solution of aptamers to RSV with a concentration of 17.5 nM
Baixar (189KB)
