Optimization of biosynthesis of butyric acid from glucose through the inverted fatty acid β-oxidation pathway by recombinant Escherichia coli strains

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Abstract

The biosynthesis of butyric acid from glucose though the inverted fatty acid β-oxidation by recombinant Escherichia coli strains was optimized. The increased yield of the target compound was achieved resulting from the plasmid expression of atoB, fadB and fadE/fabI genes in the core strain MG∆4 PL-tesB ΔyciA (MG1655 ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDj10-tesB, ∆yciA). The positive effect of enforced ATP hydrolysis on microaerobic conversion of carbohydrate substrate to the final product by the recombinants was demonstrated. Activation of the futile cycle of pyruvate-phosphoenolpyruvate-pyruvate, due to the increased expression of the ppsA gene, ensured a marked increase in glucose consumption by the recombinants and led to an increase in the molar yield of butyric acid up to 39.5%. When the components of the H+-ATP synthase complex were uncoupled resulting from the deletion of atpFH genes, the molar yield of butyric acid from glucose demonstrated by the strain forming butyryl-CoA by the action of enoyl-ACP reductase FabI reached 46%.

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About the authors

A. Yu. Gulevich

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Author for correspondence.
Email: andrey.gulevich@gmail.com
Russian Federation, Moscow, 117312

A. Yu. Skorokhodova

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: andrey.gulevich@gmail.com
Russian Federation, Moscow, 117312

V. G. Debabov

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: andrey.gulevich@gmail.com
Russian Federation, Moscow, 117312

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