Characteristics of Bacillus cereus complex Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks During Climate Change

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Abstract

Strains of Bacillus genus were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the group Bacillus cereus complex. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differentiate the studied strains from the B. anthracis species, to show their intraspecific genetic differences and the degree of relationship. Whole genome sequencing was carried out, based on the data of which MLST genotyping was carried out, which revealed 2 known sequence types and one new one, described for the first time in this work. The results obtained are of practical importance and are extremely interesting from the point of view of the evolution and phylogeography of the B. cereus complex group, since the fact that strains were isolated from permafrost suggests that their age may be much older than expected.

About the authors

Y. O. Goncharova

State Research Center for Applied Microbiology and Biotechnology

Author for correspondence.
Email: iulia.belay@yandex.ru
Russia, 142279, Moscow region, Obolensk

V. S. Timofeev

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

A. V. Brushkov

Moscow State University M.V. Lomonosov; Tyumen State University

Email: ignatov@obolensk.org
Russia, 119991, Moscow; Russia, 625003, Tyumen

T. B. Kravchenko

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

I. V. Bahtejeva

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

G. M. Titareva

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

V. I. Solomentsev

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

A. A. Sizova

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

A. G. Bogun

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

K. V. Khlopova

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

R. I. Mironova

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

V. V. Evseeva

State Research Center for Applied Microbiology and Biotechnology

Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk

S. G. Ignatov

State Research Center for Applied Microbiology and Biotechnology; Moscow State University M.V. Lomonosov

Author for correspondence.
Email: ignatov@obolensk.org
Russia, 142279, Moscow region, Obolensk; Russia, 119991, Moscow

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