ABA-degrading strains of bacteria of the genus Pseudomonas and their influence on wheat growth

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Three new representatives of the genus Pseudomonas have been identified that are capable of utilizing abscisic acid and positively influencing the growth and development of plants. Their physiological and biochemical properties have been studied. Based on the analysis of the 16S rRNA gene, they were identified as P. veronii IB K11-1 (99.86% similarity), P. frederiksbergensis IB Ta10m (100%), strain TaE2 was assigned to Pseudomonas sp. It was found that bacteria, when growing on a mineral-salt medium with ABA, reduced the hormone content by 50-60% with an increase in population density by two orders of magnitude. In a laboratory experiment, it was shown that the introduction of bacterial biomass (108 CFU/g of substrate) into the rhizosphere of wheat plants 10 days after treatment led to a decrease in the abscisic acid content in the roots by 18–30% and an increase in plant weight by up to 30%. Thus, new strains of growth-stimulating ABA-degrading bacteria have been identified and characterized for the first time, which may be promising for the creation of biological products that increase plant resistance to biotic and abiotic stresses.

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A. Ryabova

Ufa Federal Research Center of the Russian Academy of Sciences RAS

Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

L. Kuzmina

Ufa Federal Research Center of the Russian Academy of Sciences RAS

编辑信件的主要联系方式.
Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

E. Gilvanova

Ufa Federal Research Center of the Russian Academy of Sciences RAS

Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

N. Galimsyanova

Ufa Federal Research Center of the Russian Academy of Sciences RAS

Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

E. Martynenko

Ufa Federal Research Center of the Russian Academy of Sciences RAS

Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

L. Vysotskaya

Ufa Federal Research Center of the Russian Academy of Sciences RAS

Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

G. Kudoyarova

Ufa Federal Research Center of the Russian Academy of Sciences RAS

Email: alenarya@rambler.ru

Ufa Institute of Biology

俄罗斯联邦, Ufa, 450054

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2. Fig. 1. ABA content (ng/g fresh weight) in the roots (a) and the weight of wheat plants (b) 10 days after planting three-day-old seedlings in pots and adding a suspension (up to 108 CFU/g soil) of the ABA-degrading strains Pseudomonas veronii IB K11-1, P. frederiksbergensis IB Ta10m and Pseudomonas sp. IB TaE2 to the rhizosphere; control – non-inoculated plants. Mean values ​​± standard errors are shown. Different letters indicate significantly different values ​​(P ≤ 0.05, ANOVA, Duncan's test).

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3. Fig. 2. Auxin content (ng/ml) in the culture fluid of Pseudomonas veronii IB K11-1, P. frederiksbergensis IB Ta10m and Pseudomonas sp. IB TaE2 strains on King B medium on the 3rd day of cultivation. Designations as in Fig. 1.

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