Preparation of Phage Antibodies to Heat Shock Proteins and Studying the Dynamics of their Accumulation in Mice with Xenotransplant Tumors

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Abstract

Using a naïve human scFv phage library, affinity selection of miniantibodies specific to heat shock proteins isolated from MH22a hepatoma cells and Sp2/0-Ag14 plasmacytoma cells was carried out. Using the obtained phage antibodies by dot-immunoassay and enzyme-linked immunosorbent assay, the dynamics of the concentration of heat shock proteins in the blood serum of mice with implanted tumor cells of the MH22a line was studied. Starting from the 14th day after xenotransplantation, there was a gradual increase in the level of heat shock proteins in the blood serum. It was found that after implantation of tumor cells, tumor growth was accompanied by a significant increase in the accumulation of heat shock proteins in the blood serum. It has been shown that miniantibodies specific to heat shock proteins are an effective tool for determining and monitoring the accumulation of heat shock proteins in the blood serum of animals.

About the authors

L. A. Dykman

Institute of Biochemistry and Physiology of Plants and Microorganisms – Research Institution
Saratov Federal Scientific Centre of the Russian Academy of Sciences

Email: guliy_olga@mail.ru
Russia, 410049, Saratov

S. A. Staroverov

Institute of Biochemistry and Physiology of Plants and Microorganisms – Research Institution
Saratov Federal Scientific Centre of the Russian Academy of Sciences; Saratov State Vavilov Agrarian University

Email: guliy_olga@mail.ru
Russia, 410049, Saratov; Russia, 410012, Saratov

R. D. Vyrshchikov

Institute of Biochemistry and Physiology of Plants and Microorganisms – Research Institution
Saratov Federal Scientific Centre of the Russian Academy of Sciences

Email: guliy_olga@mail.ru
Russia, 410049, Saratov

K. K. Fursova

The Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic
Chemistry of the Russian Academy of Sciences

Email: guliy_olga@mail.ru
Russia, 142290, Pushchino

F. A. Brovko

The Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic
Chemistry of the Russian Academy of Sciences

Email: guliy_olga@mail.ru
Russia, 142290, Pushchino

D. A. Soldatov

Saratov State Vavilov Agrarian University

Email: guliy_olga@mail.ru
Russia, 410012, Saratov

O. I. Guliy

Institute of Biochemistry and Physiology of Plants and Microorganisms – Research Institution
Saratov Federal Scientific Centre of the Russian Academy of Sciences

Author for correspondence.
Email: guliy_olga@mail.ru
Russia, 410049, Saratov

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Copyright (c) 2023 Л.А. Дыкман, С.А. Староверов, Р.Д. Вырщиков, К.К. Фурсова, Ф.А. Бровко, Д.А. Солдатов, О.И. Гулий