Expression of genes regulating muscle growth in pink salmon Oncorhynchus gorbuscha (Walb.) smolts from different rivers of the White and Barents Sea basins

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Abstract

The expression of genes encoding myogenic regulatory factors – Myf5, MyoG, MyoD1a, MyoD1b, heavy (MyHC) and light (mlc2) myosin chains, as well as myostatin (Mstn1a) – was studied in pink salmon Oncorhynchus gorbuscha (Walb.) smolts caught in rivers during the period of migration for fattening to the White and Barents Seas. It was found that individuals from the Keret River had higher expression levels of the mlc2, MyHC, MyoD1a, MyoD1b and MyoG genes compared to those in the Varzuga River. It was shown that the smolts from the rivers of the White Sea basin – Indera, Keret and Umba, had higher expression values of the MyoD1b and MyoG genes, and in the Varzuga River – Myf5, in contrast to fish from the Voronya River of the Barents Sea basin. The highest level of expression of the Mstn1a gene was detected in pink salmon in the Voronya River. The obtained results indicate differences in the regulation of myogenesis in pink salmon smolts depending on the temperature and feeding conditions of habitat in different rivers.

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N. S. Shulgina

Institute of Biology – a separate subdivision of the FSBI FRC “Karelian Research Center of the Russian Academy of Sciences”

Author for correspondence.
Email: Shulgina28@yandex.ru
Russian Federation, Pushkinskaya str., 11, Petrozavodsk, 185910

M. V. Kuznetsova

Institute of Biology – a separate subdivision of the FSBI FRC “Karelian Research Center of the Russian Academy of Sciences”

Email: Shulgina28@yandex.ru
Russian Federation, Pushkinskaya str., 11, Petrozavodsk, 185910

D. A. Efremov

Institute of Biology – a separate subdivision of the FSBI FRC “Karelian Research Center of the Russian Academy of Sciences”

Email: Shulgina28@yandex.ru
Russian Federation, Pushkinskaya str., 11, Petrozavodsk, 185910

S. A. Murzina

Institute of Biology – a separate subdivision of the FSBI FRC “Karelian Research Center of the Russian Academy of Sciences”

Email: Shulgina28@yandex.ru
Russian Federation, Pushkinskaya str., 11, Petrozavodsk, 185910

N. N. Nemova

Institute of Biology – a separate subdivision of the FSBI FRC “Karelian Research Center of the Russian Academy of Sciences”

Email: Shulgina28@yandex.ru
Russian Federation, Pushkinskaya str., 11, Petrozavodsk, 185910

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2. Fig. 1. Relative expression level of genes Myf5 (a), MyoG (b), MyoD1a (c), MyoD1b (d) (UE) in pink salmon smolts from different rivers, M±SE. Differences are significant at p≤0.05: a – compared with fish from the Varzuga River, b – compared with fish from the Indera River, c – compared with fish from the Keret River, d – compared with fish from the Umba River.

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3. Fig. 2. Relative expression level of genes MyHC (a), mlc2 (b) (UE) in pink salmon smolts from different rivers, M±SE. Differences are significant at p≤0.05: a – compared with fish from the Varzuga River, c – compared with fish from the Keret River.

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4. Fig. 3. Relative expression level of the Mstn1a gene (LE) in pink salmon smolts from different rivers, M±SE. Designations as in Fig. 1.

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