Membrane-Protective effect of lipid extract of Marine Red Seaweed Ahnfeltia tobuchiensis (Kanno et Matsubara) Makienko under experimental carbone tetrachloride poisoning

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Abstract

Carbon tetrachloride (CCl4) disrupts the stable operation of biological membranes, causing disturbances in the lipid bilayer, as well as changes in the properties of membrane lipids. The protective effect of lipid complex isolated from the marine red alga Ahnfeltia tobuchiensis and the reference drug “Essentiale” was investigated on the plasma membranes of mouse erythrocytes, obtained in the modeling of toxic hepatitis induced byCCl₄. The lipid extract from A. tobuchiensis was not inferior in efficiency to the phospholipid preparation essenciale in restoring the indices of both membrane lipids and blood plasma lipids, as well as in normalizing the parameters of antioxidant protection of the organism. The membrane-protective effect of algal extract is due to its multicomponent composition including glyco- and phospholipids with predominance of polyunsaturated fatty acids of n-3 and n-6 families.

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About the authors

S. E. Fomenko

Il’ichev Pacific Oceanological Institute, Far East Branch, Russian Academy of Sciences

Author for correspondence.
Email: sfomenko@poi.dvo.ru
Russian Federation, Vladivostok, 690041

N. F. Kushnerova

Il’ichev Pacific Oceanological Institute, Far East Branch, Russian Academy of Sciences

Email: sfomenko@poi.dvo.ru
Russian Federation, Vladivostok, 690041

V. G. Sprygin

Il’ichev Pacific Oceanological Institute, Far East Branch, Russian Academy of Sciences

Email: sfomenko@poi.dvo.ru
Russian Federation, Vladivostok, 690041

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2. Fig. 1. Content of total lipids (a), neutral lipid fractions (b) and phospholipids (c) in the lipid extract of the red alga Ahnfeltia tobuchiensis. OL – total lipids, GL – glycolipids, PL – phospholipids, NL – neutral lipids, DAG + MAG – diacylglycerols + monoacylglycerols, SS – free sterols, FFA – free fatty acids, TAG – triacylglycerols, ES – sterol esters, PC – phosphatidylcholine, PG – phosphatidylglycerol, PE – phosphatidylethanolamine, PI – phosphatidylinositol, PS – phosphatidylserine.

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3. Fig. 2. Effect of lipid extract of Ahnfeltia and Essentiale on parameters of antioxidant system of blood plasma of mice under CCl₄ intoxication. MDA – malondialdehyde, ARA – antiradical activity, SOD – superoxide dismutase, Es – Essentiale, An – Ahnfeltia. Designations are statistically significant: * – p <0.05; ** – p <0.01; *** – p <0.001 – when compared with control. + – p <0.05; ++ – p <0.01; +++ – p <0.001 – when compared with group 3 (CCl₄ withdrawal) for Fig. 2, 3.

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4. Fig. 3. The effect of lipid extract of Ahnfeltia and Essentiale on the lipid composition of blood plasma in mice intoxicated with CCl₄.

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