Dysregulation of Porphyromonas gingivalis Agmatine Deiminase Expression in Alzheimer’s Disease


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Abstract

Background:Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, with a significant burden on global health. AD is characterized by a progressive cognitive decline and memory loss. Emerging research suggests a potential link between periodontitis, specifically the presence of oral bacteria such as Porphyromonas gingivalis (P. gingivalis), and AD progression. P. gingivalis produces an enzyme, Agmatine deiminase (AgD), which converts agmatine to N-carbamoyl putrescine (NCP), serving as a precursor to essential polyamines. Recent studies have confirmed the correlation between disruptions in polyamine metabolism and cognitive impairment.

Objective:This study aims to investigate the dysregulation of P. gingivalis Agmatine deiminase (PgAgD) in the context of AD.

Methods:Saliva samples were collected from a total of 54 individuals, including 27 AD patients and 27 healthy controls. The expression of the PgAgD gene was analyzed using quantitative Real-- Time PCR.

Results:The results showed a significant decrease in PgAgD gene expression in the saliva samples of AD patients compared to healthy controls. This downregulation was found in AD patients with advanced stages of periodontitis. Additionally, a correlation was observed between the decrease in PgAgD expression and the 30-item Mini-Mental State Examination (MMSE) score.

Conclusion:These findings suggest that measuring PgAgD expression in saliva could be a noninvasive tool for monitoring AD progression and aid in the early diagnosis of patients with periodontitis. Further research is needed to validate our results and explore the underlying mechanisms linking periodontitis, PgAgD expression, and AD pathophysiology.

About the authors

Asma Hamdi

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Email: info@benthamscience.net

Sana Baroudi

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Email: info@benthamscience.net

Alya Gharbi

Neurology Department, Razi University Hospital

Email: info@benthamscience.net

Wafa Babay

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Email: info@benthamscience.net

Ahmed Laaribi

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Email: info@benthamscience.net

Imene Kacem

Neurology Department, Razi University Hospital

Email: info@benthamscience.net

Saloua Mrabet

Neurology Department, Razi University Hospital

Email: info@benthamscience.net

Ines Zidi

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Email: info@benthamscience.net

Naouel Klibi

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Email: info@benthamscience.net

Riadh Gouider

Neurology Department, Razi University Hospital

Email: info@benthamscience.net

Hadda-Imene Ouzari

Laboratory of Microorganisms and Active Biomolecules (LR03ES03), Faculty of Sciences of Tunis, University of Tunis El Manar

Author for correspondence.
Email: info@benthamscience.net

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