Mechanisms of Control by Pseudomonas fluorescens of Barley Root Rot Caused by Fusarium culmorum

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Abstract

In this paper, we found out why in the presence of the rhizobacterium Pseudomonas fluorescens 2137, the intensity of barley fusarium root rot caused by Fusarium culmorum 30 decreases, if there is no obvious decrease in the amount of fungus in the roots in the presence of the bacterium. It has been suggested that (1) the presence of rhizobacteria stimulates the defence reactions in barley, (2) rhizobacteria reduces the production of trichothecene toxins by F. culmorum 30, a known factor of aggressiveness of the fungus. The responses of barley to the colonization of its roots by fungus and bacteria were studied in sterile vermiculite for 11 days by the intensity of expression of the LOX, PAL, PR4 and PR1 genes. The production of F. culmorum 30 trichothecene toxins was evaluated by the expression level of the TRI13 gene. As a result, it was found that P. fluorescens 2137 induced the expression of all studied defence genes already in diurnal barley roots, but only in the presence of F. culmorum 30. In the presence of the bacterium, the expression level of the TRI13 gene did not decrease, however, a decrease in the number of diseased plants suggests that P. fluorescens 2137 is capable of detoxifying trichothecene toxins produced by the fungus or inducing this ability in barley.

About the authors

V. Yu. Shakhnazarova

All-Russian Research Institute for Agricultural Microbiology; Saint Petersburg State University

Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg; Russia, 199034, Saint Petersburg

D. S. Syrova

All-Russian Research Institute for Agricultural Microbiology

Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg

M. I. Lebedinskii

All-Russian Research Institute for Agricultural Microbiology; Saint Petersburg State University

Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg; Russia, 199034, Saint Petersburg

N. A. Vishnevskaya

All-Russian Research Institute for Agricultural Microbiology

Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg

A. I. Shaposhnikov

All-Russian Research Institute for Agricultural Microbiology

Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg

E. V. Borodina

All-Russian Research Institute for Agricultural Microbiology

Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg

O. K. Strunnikova

All-Russian Research Institute for Agricultural Microbiology

Author for correspondence.
Email: olgastrunnikova@rambler.ru
Russia, 196608, Saint Petersburg

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Copyright (c) 2023 В.Ю. Шахназарова, Д.С. Сырова, М.И. Лебединский, Н.А. Вишневская, А.И. Шапошников, Е.В. Бородина, О.К. Струнникова