电邮这篇文章 Optimization of Biolistic Transformation Parameters for Nicotiana tabacum
- 作者: Davlekamova A.A.1, Zubritsky A.V.1, Timofeeva T.A.1, Yakovleva I.V.1, Kamionskaya A.M.1
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隶属关系:
- Skryabin Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
- 期: 卷 59, 编号 3 (2023)
- 页面: 289-294
- 栏目: Articles
- URL: https://cardiosomatics.ru/0555-1099/article/view/674623
- DOI: https://doi.org/10.31857/S0555109923030054
- EDN: https://elibrary.ru/AZNGOK
- ID: 674623
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详细
Biolistics is one of the widely used methods to deliver nucleic acids into plant cells. In this work, we optimized the protocol for biolistic transformation of Nicotiana tabacum with the gene gun PDS-1000/He Biolistic Particle Delivery System. The Green Fluorescent Protein (GFP) gene was used as a marker. The optimal parameters for the transformation of N. tabacum leaf cells were determined as: pressure, 1350 psi; tungsten particle size, 1.3 μm; plasmid DNA purification method, ethanol re-precipitation. The results should be useful for the development of biolistic transformation protocols for plant cells, including application for plant genome editing of agricultural species.
作者简介
A. Davlekamova
Skryabin Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology”of the Russian Academy of Sciences
Email: timofeeva.bio@gmail.com
Russia, 117312, Moscow
A. Zubritsky
Skryabin Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology”of the Russian Academy of Sciences
Email: timofeeva.bio@gmail.com
Russia, 117312, Moscow
T. Timofeeva
Skryabin Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology”of the Russian Academy of Sciences
编辑信件的主要联系方式.
Email: timofeeva.bio@gmail.com
Russia, 117312, Moscow
I. Yakovleva
Skryabin Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology”of the Russian Academy of Sciences
Email: timofeeva.bio@gmail.com
Russia, 117312, Moscow
A. Kamionskaya
Skryabin Institute of Bioengineering, Federal Research Centre “Fundamentals of Biotechnology”of the Russian Academy of Sciences
Email: timofeeva.bio@gmail.com
Russia, 117312, Moscow
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