Antifungal and Reactivation Activity of a Novel Glycine/histidine-rich Linear Peptide from Dog-grass (Elytrigiarepens (L.) Desv. ex Nevski) EARS

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Using a combination of solid-phase extraction, affinity chromatography, and analytical reverse-phase HPLC, a new linear peptide was isolated from dog-grass (Elytrigia repens) ears, which does not contain cysteine residues. Identification of its primary structure by Edman automated degradation made it possible to reveal the presence of several polyglycine regions, each consisting of 6–8 residues, between which short fragments consisting of polar amino acid residues are localized. The C-terminal fragment of the molecule is a positively charged site enriched in arginine and histidine residues. The structural features of this peptide determine its functionality. Thus, checking the presence of antimicrobial properties in its recombinant analogue, obtained by heterologous expression in a prokaryotic system, made it possible to determine the MIC for the tested fungal cultures only at sufficiently high active concentrations (52–104 μM). However, this compound had regulatory properties: at a concentration of 25 μM, a reactivating effect was noted, which increased the level of survival of Saccharomyces cerevisiae to UV-irradiation. The data obtained expand the understanding of the functional features of plant defense peptides of an unusual structural type.

作者简介

D. Ryazantsev

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

编辑信件的主要联系方式.
Email: rea21@list.ru
Russia, 117997, Moscow

E. Khodzhaev

Lomonosov Moscow State University

Email: rea21@list.ru
Russia, 119234, Moscow

A. Kuvarina

Gause Institute New Antibiotics

Email: rea21@list.ru
Russia, 119021, Moscow

A. Barashkova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Email: rea21@list.ru
Russia, 117997, Moscow

E. Rogozhin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry; Gause Institute New Antibiotics

Email: rea21@list.ru
Russia, 117997, Moscow; Russia, 119021, Moscow

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版权所有 © Д.Ю. Рязанцев, Е.Ю. Ходжаев, А.Е. Куварина, А.С. Барашкова, Е.А. Рогожин, 2023